Full Research

StageFR
Project No.C-06
Project NameEffects of Environmental Change on the Interactions between Pathogens and Humans
Abbreviated TitleEnvironmental Diseases
Project LeaderKAWABATA, Zen’ichiro
Research AxisCirculation
URLhttp://www.chikyu.ac.jp/z/
Key WordsFreshwater ecosystem, Environmental alterations, Koi herpes virus (KHV) disease, Human life, Interactions, Model

 

○Research Subject and Objectives Research Objectives and Topics

Objectives

    Infectious disease has become a significant global environmental problem. This study investigates the emergence and spread of Koi HerpesVirus (KHV) in Lake Biwa, Japan. KHV is a pathogen responsible for episodic mass mortality of commoncarp (Cyprinus carpio carpio)  since the late1990s. The common carp is the original domesticated aquaculture species, and an important source of protein today .

    This study has three main objectives: (1) To describe Koi Herpes Virus disease ecology, including: the specific links between anthropogenic changes to freshwater ecosystems and the emergence and spread of KHV disease; the impacts of KHV disease on local ecosystem services; the social and cultural attempts to address KHV disease; and the environmental changes associated with human adaptation; (2) To describe a general model of linkage between environments, pathogens and humans ; (3) To suggest how interactions between pathogen and humans may be modified in order to mitigate the human and environmental damages associated with infectious diseases.

 

Research Methods and Organization

    Fields surveys areconducted at Lake Biwa, Japan, and Lake Erhai, China.Laboratory work is undertaken at RIHN. Our project isorganized into five research groups, plus executive andadvisory groups, as follows: The Human AlterationsGroup investigates the effects of anthropogenicenvironmental alteration on the emergence and spread of KHV and thebehavior of its host Cyprinuscarpio carpio.The Pathogen and HostEcology Group defines the biology and ecology of KHVand carp, and so describes the environmental factorsinvolved in KHV infection and transmission.The Ecosystem ImpactsGroup examines the process of infection and theeffects of KHV disease on ecosystem functions such as materialcycling. The Economics and Culture Group investigates the losses associated with KHVdisease, including of ecosystem services or other economicand cultural phenomena, and describes the socialattempts to redress those losses. The Feedback Groupexamines the human response to losses caused by KHVdisease, and the environmental change associated withthis response. The Executive Group coordinates theactivities of each group and develops the model ofpathogen-human  interactions.Finally, an Advisory Group composed ofrecognized experts in relevant fields makes suggestionsin order to improve the research.

 

Perception and contribution to global environmental problems 

     Field surveys are being conducted primarily at Lake Biwa, Japan, where researchers from various disciplines and fishermen have accumulated much data, and at Lake Erhai, China. China is responsible for almost the entire global carp production. Lake Erhai is an inland lake in China that has never experienced an outbreak of KHV. We will create a model to predict the outbreak and spread ofKHV in Lake Biwa by accumulating and synthesizing both new and existing data from Lake Biwa.  The model will be applied to Lake Erhai to provide suggestions for how to manage the lake to lessen the probability ofan outbreak of KHV disease. The practical application of this model to such an important region is critical to attenuating the global problem of KHV disease.  The Lake Biwa model will be modified to apply to other infectious diseases in other areas, to suggest environments that might prevent the outbreak and spread of infectious disease, and to demonstrate how to facilitate the safe coexistence of humans and pathogens.

○Progress and Results in 2017

Main results to date

 

1) We found that gentle gradient lakeshores provide a wide range of thermal conditions, suggesting that fish can choose tempertures to alleviate stress associated with unfavorable water temperatures, and thus reduce susceptibility to KHV (Yamanaka et al., 2010).

2) We established an innnovative method to quantitatively detect KHV in natural environments (Minamoto et al., 2009;Honjo et al., 2010). The method revealed that since it was first detected in 2003, KHV is now found throughout the Lake Biwa ecosystem, including in plankton and sediment, lagoons and ponds, and now in almost all the rivers in Japan. We demonstrated that it is impossible to eliminate KHV, but that precautionary environmental management can eliminate

 "fertile" disease environments.

3) We have developed a preliminary break-through method allowing determination of the number and location of carp in their natural environments.

4) We found that breeding habitats can became hot spots for transmission of infectious diseases if hosts aggregate for mating and pathogen activation occurs during the host breeding season (Uchii et al., 2011) .

5) We developed a non-invasive method (i.e. a method that does not require handling fish) to quantify how water conditions stress carp. Using this method we found that changes in water temperature do induce stress.

6) We applied a Based on our assessment of KHV disease in Lake Biwa, we applied our conceptual model of linked environment-pathogen-human interactions to Lake Erhai, China, schistosomiasis in Kenya, fish diseases in the Ping River at Chaing Mai, Thailand, and Legionella disease (Yamaguchi et al., 2010), MRSA, Norovirus disease, and nontuberculous mycobacteria disease (Ichijo et al., 2010) in Japan. These applications helped us understand how pathogens interact with humans and suggested enviromental conditions that might prevent disease outbreaks and spread as well asstrategies for safe coexistence of humans with pathogens.

7) By combining the results from each work group, ranging from molecular biology to envirnmental sciences to human society, we are providing evidence to support the hypothesis that anthropogenic encironmental changes promote disease outbreaks.

8) We have presented our findings at national and international conferences of the linkages between the environment, pathogens and humans, emphsizing their significance to preventionand control of infectious disease.

○Project Members

KAWABATA, Zen’ichiro ( Research Institute for Humanity and Nature,,Professor,Management )

ASANO, Kota ( Graduate School of Human and Environmental Studies, Kyoto University,Professor,Model for Economical Effects )

ABE, Akira ( Research Institute for Humanity and Nature,,Research Fellow,Sociology Ethics )

ITAYAMA, Tomoaki ( Research Institute for Humanity and Nature,Research Fellow,Nano-technological Measurements )

OHMORI, Koji ( Center for Marine Environmental Studies, Ehime University,Associate Professor,Environmental Alteration )

OKUDA, Noboru ( Center for Ecological Research, Kyoto University,Associate Professor,Food Web Analysis )

NASU, Masao ( Graduate School of Pharmaceutical Sciences, Osaka University,,Professor,Genome Analysis of Pathogens )

MATSUOKA, Masatomi ( Asahi Fishery Cooperative, Shiga Prefecture,,Secretary,Food Culture )

MINAMOTO, Toshifumi ( Research Institute for Humanity and Nature,Senior Researcher,,Detection of KHV )

YAMANAKA, Hiroki ( Dept. Environmental Solution Technology Fac. Science & Technology, Ryukoku Univ.,Lab Assistant,Fish Habitats )

KAKEHASHI, Masayuki ( Graduate School of Health Science, Hiroshima University,,Professor,Model for Epidemics )

KONG, Hainan ( Shanghai Jiao Tong University,Professor,Lake Management )

WU, Deyi ( Shanghai Jiao Tong University,Associate Professor,Lake Management )

IBUKI, Naomi ( Research Institute for Humanity and Nature,Research Associate,Secretariat )

ICHIJO,Tomoaki ( Research Institute for Humanity and Nature,Research Fellow,Legionella Disease )

UCHII, Kimiko ( Laboratoire Microorganismes,Research Fellow,Atibody Titer Measurement )

ENDO, Takahiro ( University of Tsukuba Graduate School of Life and Environmental Sciences,Associate Professo )

OKUMIYA, Kiyohito ( Research Institute for Humanity and Nature,Associate Professor,Human Health )

KOHMATSU, Yukihiro ( Research Institute for Humanity and Nature,,Assistant Professor,Stress of Fish )

KONDOH, Michio ( Ryukoku University,Lecturer,System Stability Analysis )

SAKAI, Yoichiro ( Center for Ecological Research, Kyoto University,Graduate Student,Fish Habitat )

SAKAMOTO, Ryota ( Research Institute for Humanity and Nature,,Research Fellow,Public Health )

SHIBATA, Junya ( Ehime University. Global COE Program.,Global COE Researcher,Ecosystem Analysis Division )

SHIRAE, Yusuke ( Graduate School of Human and Environmental Studies, Kyoto University,Graduate student,Model for Economical Effects )

SUZUKI, Arata ( Research Institute for Humanity and Nature,Research Fellow,Stress on Fish )

TAKAHARA,Teruhiko ( Research Institute for Humanity and Nature,Research Fellow,Metabolic Physiology )

TANAKA, Takuya ( Center for Ecological Research, Kyoto University,Research Fellow,Environmental Studies )

TANAKA,Nobuyuki ( National Institute for Environmental Studies,Research Assistant,Nano-technological Measurements )

TAYASU, Ichiro ( Center for Ecological Research, Kyoto University,Associate Professor,Stable Isotopic Analysis )

NAKANO, Takanori ( Research Institute for Humanity and Nature,Professor,Stable Isotopic Analysis )

NAKANO,Shinich ( Center for Ecological Research, Kyoto University,Professor,Food Web Analysis )

PARK,Ho-Dong ( Faculty of Science, Shinshu University,Professor,Water Pollution )

FUMA, Shoichi ( National Institute of Radiological Sciences Environmental Radiation Effects Research Group,Team Reader,Environmental impact assessment/environmental policy )

HONJO, Mie ( Research Institute for Humanity and Nature,Research Fellow,Detection of KHV )

MASAGO, Yoshifumi ( Tohoku University Graduate School of Engineering Department of Civil and Environmental Engineering,Assistant Professor,Environmental dynamic analysis ,Environmental impact )

MATSUI, Kazuaki ( Faculty of Science and Technology, Kinki University ,,Assistant Professor,Antibody Titer Measurement )

MIKI, Takeshi ( Institute of Oceanography, National Taiwan University,Assistant Professor,Mathematical Models of Epidemics )

MIURA, Takayuki ( Environmental Innovation Engineering Department, Faculty of Engineering, Hokkaido University,Postdoctoral fellow,Civil and Environmental Systems )

DIVERSITAS ( International Programme of Biodiversity Science Members,Secretariat in France, 11 members of 9countries,Advisory for International Symposium )

YACHI, Shigeo ( Kyoto University Center for Ecological Research,Associate Professor,Environmental impact assessment/environmental policy )

YASUNAGA, Teruo ( Genome Information Research Center , Research Institute for Microbial Diseases, Osaka University,Professor,Genome Analysis )

YONEKURA, Ryuji ( Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments,Researcher,Stress on Fish )

TELSCHOW,Arndt ( Westfalian Wilhelms University,Muenster, Germany,Postdoctoral Research Fellow,Mathematical Model )

Charles Lange ( National Museums of Kenya,Senior Researcher )

SOTO, Doris ( Fishery Department, FAO, UN, Rome, Italy,Senior Fishery Resources Officer,,Resource Analysis )

RAPPORT, David J. ( EcoHealth Consulting,Canada,Director,Eco Health )

MAFFI, Luisa ( Terralingua (International NGO) Canada,Director,Sociological Analysis )

KOTLER, Moshe ( Medical School, The Hebrew University?Hadassah Jerusalem, Israel,Professor,Molecular Study on KHV )

Niwooti Whangchai  ( Maejo University,Professor,Fisheries Technology and Aquatic Resources )

NAIMAN,Robert ( University of Washington,Professor,Fish Habitat )

MARAKKALE, Manage ( University of Sri Jayewardenepura University, Sri Lanka,Senior Lecturer,Eutrophication )

○Future Themes

Research objectives in 2011

 

1) Refine a method to quantify carp spatially and temporally and innovative method to describe our aonceptual model of environment-pathogen-human linkages including a deveropment of micro-device capable of in-situ measurment of KHV incidence and infectivity.

2) Determine the environmental factors involved in KHV abundance and its infectivity, and in carp population dencity and its susceptibility.

3) Conduct controlled experiments to reveal the relationship between water temperature, carp stress and susceptibility to KHV.

4) Assess the economic and cultural impacts of carp die-offs.

5) Apply the conceptual model of environment-pathogen-human linkage to other infectious diseases; KHV disease in Lake Erhai, China, Schistosomiasis in Kenya, fish deseases in the Ping River at Chaing Mai, Thailand, and Legionela disease, MRSA, Norovirus desease, and nontuberculous mycobacteria desease in Japan, and then describe the common parameters of KHV disease and other infectious diseases.

6) Continue to explore evidence that anthropogenic environmental changes can mediate disease outbreaks.

7) Synthesis of results of the work groupes in order to develop a set of recommendations desined to minimize the emergence and facilitate the safe coexistence of humans with pathogens.

8) Publish our results in international journals and books in order to disseminate the concepts and practical measures that can aid the control of "fertile" disease environments.

9) Organize a national and international symposium on environment-pathogen-human linkage to develop a network for this field of study.

 

  

Scheduled Research Activities in 2010

 

1) Develop a method to quantitatively detect KHV in sediment, organisms and other elements in aquatic ecosystems.

2) Clarify the distribution of infectious KHV in Lake Biwa.

3) Develop a micro-device to measure the quantity and infectivity of KHV in situ.

4) Determine the environmental factors involved in KHV dynamics and infectivity.

5) Use outdoor experimental tanks to define optimum water temperature for carp.

6) Describe the environmental characteristics of the places where KHV and carp interact, and clarify the behavior of the KHV-infected carp in order to reveal the locations where infection likely occurs .

7) Conduct controlled experiments to reveal the relationship water temperature and carp stress and susceptibility to KHV.

8) Demonstrate the ecological effects of carp on species composition in experimental ponds.

9) Evaluate the cultural and nutritional value of carp as a human food.

10) Assess the economic and cultural impacts of carp die-offs.

11) Create a preliminary model of the interactions between environmental change, KHV and humans.

12) Survey the spatial and temporal distribution of water temperature in Lake Erhai in order to establish the applicability of Lake Biwa findings to Lake Erhai.

13) Describe the common parameters of KHV andother infectious diseases.

14) Promote collaboration with the DIVERSITAS program of international biodiversity science.

15) Develop a set of recommendations to prevent or minimize the emergence and spread of infectious diseases.

Books

【Authored/Co-authored】

Abe A, 2011,03 The greeting of solidarity - Thought of Rorty and hope. Seikatsushoin, Tokyo, Japan, 260pp. (in Japanese)

Morotomi T, Asano K, Mori A, 2008,06 The lecture of environmental economics - Aim at sustainable development. Yuhikaku Publishing, Tokyo, Japan, 296pp. (in Japanese)

Sekihara T, Takeuchi Y, Kakehashi M, Yamamura N, 2007,03 A guide to ethics biology. Gendaitoshi, Kanagawa, Japan, 319pp. (in Japanese)

【Chapters/Sections】

Okuda N, Takeyama T, Komiya T, Kato Y, Okuzaki Y, Karube J, Sakai Y, Hori M, Tayasu I, & Nagata T, 2012 A food web and its long-term dynamics in Lake Biwa: a stable isotope approach. In: Lake Biwa: Relationship between Human and Nature. Nakamura M. et al (ed.) Springer Academic, Amsterdam. in press

Uchii K, 2011,10 Use and damage of an alien species in fisheries. Nishikawa U, Miyashita T, (ed.) Influence - on alien species-biodiversity and human society . Shokabo, Tokyo, Japan. (in Japanese)

Miki T, 2011,03 Group biology of a microbe. Osono T, Kagami M, (ed.) Ecology of a microbe. Kyoritu, Tokyo, Japan. (in Japanese)

Meitei H, Manishankar K, Kakehashi M, Rao ASRS, 2011 Estimating AIDS Related Deaths in India: A Back Calculation Approach. Somayajulu UV, Prakasam CP, Audinarayana N, Vaidyanathan KE (ed.) Health, Poverty and Human Development Perspectives and Issues. Global Research Publications, New Delhi, pp.235-244. Chapter 11

Kakehashi M, 2010,12 Mathematical models of infectious diseases. Ishikawa H, Kuroiwa T, Shiomi M, Matsumoto T, Mori T, Yasugi S, Yamamoto M (ed.) The Unabridged of Biology. Tokyo Kagaku Dojin, Tokyo, pp.268. (in Japanese)

Kawabata Z, 2010,10 Koi herpes virus disease. Narifumi Tachimoto and Toshitaka Hidaka(supervised), Research Institute for Humanity and Nature(RIHN) (ed.) The RIHN Encyclopedia of Global Environmental Studies. Kobun-do, Tokyo, pp.284-285. (in Japanese)

Kawabata Z, 2010,10 Freshwater bio-divesity. Narifumi Tachimoto and Toshitaka Hidaka(supervised), Research Institute for Humanity and Nature(RIHN) (ed.) The RIHN Encyclopedia of Global Environmental Studies. Kobun-do, Tokyo, pp.168-169. (in Japanese)

Kawabata Z, 2010,10 Horizontal Gene Transsfer. Narifumi Tachimoto and Toshitaka Hidaka(supervised), Research Institute for Humanity and Nature(RIHN) (ed.) The RIHN Encyclopedia of Global Environmental Studies. Kobun-do, Tokyo, pp.72-73. (in Japanese)

Okuda N, 2010,08 Analysis of a stable isotope. Tsukamoto K, (ed.) The foundation of fishes ecology. Kouseisha-Kouseikaku Corporation, Tokyo, Japan, pp.110-119. (in Japanese)

Kakehashi M, 2009,12 Mathematical modeling on the spread of infectious diseases ( II. Mathematical science in life sciences, Chap. 6 Mathematical science in medicine). Hironaka H (Representative editor) (ed.) The second edition Encyclopedia in Modern mathematical sciences. Maruzen, Tokyo, pp.5. (in Japanese)

Watanabe M, Asano K, 2009,08 Bargaining ability and social public welfare beyond administrative boundaries of voluntary agreement. Agriculture and Forestry Statistics Publishing Inc. Agriculture and Forestry Statistics Publishing Inc, Tokyo, Japan, pp.185-198. (in Japanese)

Matsushita K, Asano K, 2009,08 The independence agreement for the environmental impact mitigation to an agricultural public water area. Circulation practical use and management of resources in a rural area. Agriculture and Forestry Statistics Publishing Inc, Tokyo, Japan, pp.173-184. (in Japanese)

Nishiura H, Kakehashi M, and Inaba H, 2009,06 Two critical issues in quantitative modeling of communicable diseases: Inference of unobservables and dependent happening. Chowell G, Hyman JM, Bettencourt LMA, Castillo-Chavez C (Eds) (ed.) Mathematical and Statistical Estimation Approaches in Epidemiology. Springer, Dordrecht Heidelberg London New York, pp.35.

Kakehashi M, 2008,09 Theoretical epidemiology:Analysis of the infection fashion in a mathematical model. Mathematical biology of a "number". Japanese Society for Mathematical Biology. Kyoritsu Publisher, Tokyo, Japan, pp.117-134. (in Japanese)

Okuda N, 2008,03 Soundness evaluation of the coastal ecosystem based on food web analysis. Nagata, Miyazima, (ed.) Valley environmental evaluation and a stable isotope. Kyoto University Press, Kyoto, Japan. (in Japanese)

Kakehashi M, 2007,12 . Inaba H, (ed.) Perspectives of modern human demography of infectious diseases - influence and mathematical theory of infectious diseases -. Minerva Library, pp.196-217. (in Japanese)

Editing

【Editing / Co-editing】

Asano K, (ed.) 2009,11 Preservation and evaluation of natural capital. Series of environmental governance, 5. Minerva Library, Kyoto, Japan, 271pp. (in Japanese)

Papers

【Original Articles】

Honjo M N, Minamoto T, Kawabata Z, 2012,03 Reservoirs of Cyprinid herpesvirus 3 (CyHV-3) DNA in sediments of natural lakes and ponds. Veterinary Microbiology 155(2-4):183-190. DOI:10.1016/j.vetmic.2011.09.005 (reviewed).

Baba T, Inoue N, Yamaguchi N, Nasu M, 2012,02 Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining. (reviewed).

Zhang R, Ichijo T, Hu YY, Zhou HW, Yamaguchi N, Nasu M, Chen GX, 2012,01 A ten years (2000-2009) surveillance of resistant Enterobacteriaceae in Zhejiang Province, China. Microbial Ecology in Health and Disease. DOI:10.3402/mehd.v23i0.11609 (reviewed).

Minamoto T, Wada E, Shimizu I, 2012 A new method for random mutagenesis by error-prone polymerase chain reaction using heavy water. Journal of Biotechnology 157(1):71-74. DOI:10.1016/j.jbiotec.2011.09.012 (reviewed). in press

Minamoto T, Honjo M N, Yamanaka H, Uchii K, Kawabata Z, 2012 Nationwide Cyprinid herpesvirus 3 contamination in natural rivers of Japan. Research in Veterinary Science. DOI:10.1080/09291016.2010.537447 (reviewed). in press

Nakazawa T, Ushio M, and Kondoh M, 2012 Scale dependence of predator-prey mass ratio: determinants and applications. Advances in Ecological Research 45:270-299. DOI:10.1016/B978-0-12-386475-8.00007-1 (reviewed). in press

Minamoto T, Yamanaka H, Takahara T, Honjo M N, Kawabata Z, 2012 Surveillance of fish species composition using environmental DNA. Limnology. DOI:10.1007/s10201-011-0362-4 (reviewed). in press

Zhang R, Ichijo T, Huang YL, Cai JC, Zhou HW, Yamaguchi N, Nasu M, Chen GX, 2011,12 High Prevalence of qnr and aac(6’)-Ib-cr Genes in Both Water-Borne Environmental Bacteria and Clinical Isolates of Citrobacter freundii in China. Microbes and Environments. DOI:10.1264/jsme2.ME11308 (reviewed).

Kawabata Z, Minamoto T, Honjo M N, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Asano K, Itayama T, Ichijo T, Omori K, Okuda N, Kakehashi M, Nasu M, Matsui K, Matsuoka M, Kong H, Takahara T, Wu D, Yonekura R, 2011,10 Environment-KHV-carp-human linkage as a model for environmental diseases. Ecological Research 26(6):1-6. DOI:10.1007/s11284-011-0881-9 (reviewed). Special feature

Takahara T, Yamanaka H, Suzuki A A, Honjo M N, Minamoto T, Yonekura R, Itayama T, Kohmatsu Y, Ito T, Kawabata Z, 2011,10 Stress response to daily temperature fluctuation in common carp Cyprinus carpio L. Hydrobiologia 675(1):65-73. DOI:10.1007/s10750-011-0796-z (reviewed).

Fuma S, Kawaguchi I, Kubota Y, Yoshida S, Kawabata Z, Polikarpov GG, 2011,09 Effects of chronic γ-irradiation on the aquatic microbial microcosm: equi-dosimetric comparison with effects of heavy metals. Journal of Environmental Radioactivity. DOI:10.1016/j.jenvrad.2011.09.005 (reviewed).

Iwamoto T, Nakajima C, Nishiuchi Y, Kato T, Yoshida S, Nakanishi N, Tamaru A, Tamura Y, Suzuki Y, Nasu M, 2011,07 Genetic diversity of Mycobacterium avium subsp. hominissuis strains isolated from humans, pigs, and human living environment. Infection, Genetics and Evolution. DOI:10.1016/j.meegid.2011.06.018 (reviewed).

Minamoto T, Honjo M N, Yamanaka H, Tanaka N, Itayama T, Kawabata Z, 2011,06 Detection of cyprinid herpesvirus-3 DNA in lake plankton. Res. Vet. Sci 90(3):530-532. DOI:10.1016/j.rvsc.2010.07.006 (reviewed).

Uchii K, Telschow A, Minamoto T, Yamanaka H, Honjo M N, Matsui K, Kawabata Z, 2011,02 Transmission dynamics of an emerging infectious disease in wildlife through host reproductive cycles. ISME Journal 5(2):244-251. DOI:10.1038/ismej.2010.123 (reviewed).

Yamanaka H, Sogabe A, Handoh I C, Kawabata Z, 2011,01 The effectiveness of clove oil as an anaesthetic on adult common carp, Cyprinus carpio L. Journal of Animal and Veterinary Advances 10(2):210-213. DOI:10.3923/javaa.2011.210.213 (reviewed).

Shibata J, Karube Z, Oishi M, Yamaguchi M, Goda Y, & Okuda N, 2011,01 Physical structure of habitat network differently affects migration patterns of native and invasive fishes in Lake Biwa and its tributary lagoons: Stable isotope approach. Population Ecology 53(1):143-153. DOI:10.1007/s10144-010-0213-x (reviewed).

Yamamura N, Telschow A, Uchii K, Kawabata Z, 2011,01 A basic equation for population dynamics with destruction of breeding habitats and its application to outbreak of cyprinid herpesvirus 3 (CyHV-3). Ecological Research 26(1):181-189. DOI:10.1007/s11284-010-0775-2 (reviewed).

Perrings C, Naeem S, Ahrestani F, Bunker D E, Burkill P, Ganziani G, Elmqvist T, Ferrati R, Fuhrman J, Jaksic F, Kawabata Z, Kinzig A, Mace G M, Milano F, Mooney H, Prieur-Richard A H, Tschirhart J, and Weisser W, 2010,12 Biodiversity Transcends Services. Science 330(6012):1744-1745. DOI:10.1126/science.330.6012.1744-c (reviewed).

Sato M, Kawaguchi Y, Yamanaka H, Okunaka T, Nakajima J, Mitani Y, Shimatani Y, Mukai T, Onikura N, 2010,11 Predicting the spatial distribution of the invasive piscivorous chub (Opsariichthys uncirostris uncirostris) in the irrigation ditches of Kyushu, Japan: a tool for the risk management of biological invasions. Biological Invasions 12(11):3677-3686. DOI:10.1007/s10530-010-9762-3 (reviewed).

Kondoh M, Kato S, and Sakato Y, 2010,11 Food webs are built up with nested subwebs. Ecology 91:3123-3130. DOI:10.1890/09-2219.1 (reviewed).

Perrings C, Naeem S, Ahrestani F, Bunker D E, Burkill P, Canziani G, Elmqvist T, Ferrati R, Fuhrman J, Jaksic F, Kawabata Z, Kinzig A, Mace G M, Milano F, Mooney H, Prieur-Richard A H, Tschirhart J, and Weisser W, 2010,10 Ecosystem services for 2020. Science 330(6002):323-324. DOI:10.1126/science.1196431 (reviewed).

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, 2010,09 Rapid enumeration of respiratory active mycobacteria with fluorescent double staining. J. Microbiol. Methods 82:327-329. DOI:10.1016/j.mimet.2010.06.010 (reviewed).

Karube Z, Sakai Y, Takeyama T, Okuda N, Kohzu A, Yoshimizu C, Nagata T, & Tayasu I, 2010,07 Carbon and nitrogen stable isotope ratios of macroinvertebrates in the littoral zone of Lake Biwa as indicators of anthropogenic activities in the watershed. Ecological Research 25(4):847-855. DOI:10.1007/s11284-010-0715-1 (reviewed).

Miki T, Ushio M, Fukui S, and Kondoh M, 2010,07 Functional diversity of microbial decomposers facilitates plant coexistence in a plant–microbe–soil feedback model. Proceedings of the National Academy of Sciences of the United States of America 107:14251-14256. DOI:10.1073/pnas.0914281107 (reviewed).

Yamaguchi W, Kondoh M, and Kawata M, 2010,04 Effects of evolutionary changes in prey use on the relationship between food web complexity and stability. Population Ecology 53(1):59-72. DOI:10.1007/s10144-010-0212-y (reviewed).

Yamanaka H, Kohmatsu Y, Minamoto T, Kawabata Z, 2010,04 Spatial variation and temporal stability of littoral water temperature relative to lakeshore morphometry: environmental analysis from the view of fish thermal ecology. Limnology 11(1):71-76. DOI:10.1007/s10201-009-0281-9 (reviewed).

Yamaguchi N, Ichijo T, Nasu M, 2010,04 Environmental disease: environmental alteration and infectious disease. Ecological Research. DOI:10.1007/s11284-010-0707-1 (reviewed).

Kondoh M, 2010,02 Linking learning adaptation to trophic interactions: a brain size-based approach. Functional Ecology 24(1):35-43. DOI:10.1111/j.1365-2435.2009.01631.x (reviewed).

Nakazawa T, Sakai Y, Hsieh C-H, Koitabashi T, Tayasu I, Yamamura N,& Okuda N, 2010,02 Is the relationship between body size and trophic niche position time-invariant in a predatory fish? First stable isotope evidence. PLoS ONE 5(2):e9120. DOI:10.1371/journal.pone.0009120 (reviewed).

Honjo M N, Minamoto T, Matsui K, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Iida T, & Kawabata Z, 2010,01 Quantification of cyprinid herpesvirus-3 (CyHV-3) in environmental water using an external standard virus. Applied and Environmental Microbiology 76:161-168. DOI:10.1128/AEM.02011-09 (reviewed).

Ichijo T, Yamaguchi N, Tani K, and Nasu M, 2010 Oligonucleotide probes for phylogenetic detection of waterborne bacteria. Journal of Health Science 56(3). (reviewed).  http://jhs.pharm.or.jp/data/56(3)/56_321.pdf

Chen Xuechu, Wang Xiaodong, Wu Deyi, He Shengbing, Kong Hainan, and Kawabata Z, 2009,12 Seasonal variation of mixing depth and its influence on phytoplankton dynamics in the Zeya reservoir, China. The Japanese Society of Limnology 10(3):159-165. DOI:10.1007/s10201-009-0292-6 (reviewed).

Minamoto T, Honjo M N, Kawabata Z, 2009,11 Seasonal Distribution of Cyprinid Herpesvirus 3 in Lake Biwa, Japan. Applied and Environmental Microbiology 75(21):6900-6904. DOI:10.1128/AEM.01411-09 (reviewed).

Uchii K, Matsui K, Iida T, and Kawabata Z, 2009,10 Distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp Cyprinus carpio L. Journal of Fish Diseases 32(10):857-864. DOI:10.1111/j.1365-2761.2009.01064.x (reviewed).

Watanabe M, and Asano K, 2009,09 Distribution Free Consistent Estimation of Mean WTP in Dichotomous Choice Contingent Valuation. Environmental and Resource Economics 44(1):1-10. DOI:10.1007/s10640-008-9255-3 (reviewed).

Kondoh M, and Ninomiya K, 2009,09 Food-chain length and adaptive foraging. Proceedings of the Royal Society of London. Series B 276(1670):3113-3121. DOI:10.1098/rspb.2009.0482 (reviewed).

Chen XC, Kong HN, He SB, Wu DY, Huang YY, 2009,08 Laboratory investigation of reducing two algae from eutrophic water treated with light-shading plus aeration. Chemosphere 76(9):1303-1307. DOI:10.1016/j.chemosphere.2009.05.027 (reviewed).

Yonekura R, Yamanaka H, Ushimaru A, Matsui K, Uchii K, Maruyama A, 2009,06 Allochthonous prey subsidies provide an asymmetric growth benefit to invasive bluegills over native cyprinids under the competitive conditions in a pond. Biological Invasions 11(6):1347-1355. DOI:10.1007/s10530-008-9342-y (reviewed).

Chen XC, Kong HN, He SB, Wu DY, Li CJ, and Huang XC, 2009,03 Reducing harmful algae in raw water by light-shading. Process Biochemistry 44(3):357-360. DOI:10.1016/j.procbio.2008.11.002 (reviewed).

Minamoto T, Honjo M N, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Iida T, Kawabata Z, 2009,03 Detection of cyprinid herpesvirus 3 DNA in river water during, after an outbreak. Veterinary Microbiology 135:261-266. DOI:10.1016/j.vetmic.2008.09.081 (reviewed).

Wang YH, Zhang JX, Kong HN, Inamori Y, Xu KQ, Inamori R, Kondo T, 2009,01 A simulation model of nitrogen transformation in reed constructed wetlands. Desalination 235(1-3):93-101. DOI:10.1016/j.desal.2008.01.012 (reviewed).

Kondoh M, 2008,10 Building trophic modules into a persistent food web. Proceedings of The National Academy of Sciences - PNAS 105:16631-16635. DOI:10.1073/pnas.0805870105 (reviewed).

Huang Yingying, Chen Xuechu, Kong Hainan, Li Chunjie, Ding Wei, Wang Yan, 2008,10 The effect on algae decay by aeration under light-shading condition. Environmental Pollution & Control 30:44-47. (in Chinese) (reviewed).  http://en.cnki.com.cn/Article_en/CJFDTOTAL-HJWR200810019.htm

Matsui K, Honjo M, Kohmatsu Y, Uchii K, Yonekura R, and Kawabata Z, 2008,06 Detection and significance of koi herpesvirus (KHV) in freshwater environments. Freshwater Biology 53(6):1262-1272. DOI:10.1111/j.1365-2427.2007.01874.x (reviewed).

Ichijo T, Yamaguchi N, Tani K, amd Nasu M, 2008,02 16S rRNA sequence-based rapid and sensitive detection of aquatic bacteria by on-chip hybridization following multiplex PCR. Journal of health science. 54(1):123-128. (reviewed).  http://jhs.pharm.or.jp/data/54(1)/54_123.pdf

Wang YH, Inamori R, Kong HN, Xu KQ, Inamori Y, Kondo T, Zhang JX, 2008,01 Influence of plant species and wastewater strength on constructed wetland methane emissions and associated microbial populations. Ecological Engineering 32:22-29. DOI:10.1016/j.ecoleng.2007.08.003 (reviewed).

Yao Zhihong, Kong Hainan, Jin Zhicheng, Wang Chen, Pan Wei, 2008 Improved Genetic Neural Network and Its Application in Forecasting of Rich Nourishment of Water and Blue-Green Algae. Shanghai Jiaotong Daxue Xuebao 42(2):262-265. (in Chinese) (reviewed). ISSN:1006-2467

Miki T, Ueki M, Kawabata Z, and Yamamura N, 2007,12 Long-term dynamics of catabolic plasmids introduced to a microbial community in a polluted environment: mathematical model. FEMS Microbiology Ecology 62(2):211-222. DOI:10.1111/j.1574-6941.2007.00357.x (reviewed).

Sekino T, Genkai-Kato M, Kawabata Z, Yoshida Y, Kagami M, Gurung T B, Urabe J, Higashi M, and Nakanishi M, 2007,12 Role of phytoplankton size distribution in lake ecosystems revealed by a comparison of whole plankton community structure between Lake Baikal and Lake Biwa. Limnology 8(3):227-232. DOI:10.1007/s10201-007-0218-0 (reviewed).

Yamanaka H, Y Kohmatsu, and M Yuma, 2007,08 Difference in the hypoxia tolerance of the round crucian carp and largemouth bass: implications for physiological refugia in the macrophyte zone. Ecological Research 54(3):308-312. DOI:10.1007/s10228-006-0400-0 (reviewed).

Honjo M, Matsui K, Ishii N, Nakanishi M, and Kawabata Z, 2007,01 Viral abundance and its related factors in a stratified lake. Archiv fuer Hydrobiologie 168(1):105-112. DOI:10.1127/1863-9135/2007/0168-0105 (reviewed).

Ichijo T, Yamaguchi N, Tani K, and Nasu M, 2006,06 Suspension array as the rapid and high sensitivity monitoring technique for pathogen in natural water. Society of eco-engeneering annual symposeum:49-52. (in Japanese) (reviewed).

Dudgeon D, Arthington A H, Gessner M O, Kawabata Z, Knowler D J, Leveque C, Naiman R J, Prieur-Richard A, Soto D, Stiassny M J, and Sullivan C A, 2006,05 Freshwater biodiversity: importance, threats, status and conservation challenges. The Biological Reviews 81(2):163-182. DOI:10.1017/S1464793105006950 (reviewed).

Uchii K, Matsui K, Yonekura R, Tani K, Kenzaka T, Nasu M, and Kawabata Z, 2006,04 Genetic and physiological characterization of the intestinal bacterial microbiota of bluegill (Lepomis machrochirus) with three different feeding habits. Microbial Ecology 51(3):277-284. DOI:10.1007/s00248-006-9018-z (reviewed).

Honjo M, Matsui K, Ueki M, Nakamura R, Fuhrman J A, and Kawabata Z, 2006,02 Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond. Journal of Plankton Research 28:407-412. DOI:10.1093/plankt/fbi128 (reviewed).

Nishiura H, Schwehm M, Kakehashi M, and Eichner M, 2006,01 Transmission potential of primary pneumonic plague: time inhomogeneous evaluation based on historical documents of the transmission network. Journal of Epidemiology & Community Health 60(7):640-645. DOI:10.1136/jech.2005.042424 (reviewed).

【Review Articles】

Nasu M, 2010,12 Microbial ecosystem is affected by environmental change. Antibiotics and Chemotherapy 26:2372-2376. (in Japanese) (reviewed).

Yamaguchi N, Nasu M, 2010,07 The life with a healthy microbe involving the earth is secured. The Pharmaceutical Society of Japan 46:659-663. (in Japanese) (reviewed).

Asano K, 2009,12 Happiness, Value Transformation, and New Challenges of Rural Planning. Journal of Rural Planning Association 28(3):117-120. (in Japanese) (reviewed).

Uchii K, and Kawabata Z, 2009,10 Biological invasion of a fish pathogen, cyprinid herpesvirus 3, into Japanese freshwater ecosystems.. Japanese Journal of Limnology 70(3):267-272. (in Japanese) (reviewed).

Research Presentations

【Oral Presentation】

Honjo M N, Minamoto T, Kawabata Z, Seasonal and spatial distribution of Cyprinid herpesvirus 1 and Cyprinid herpesvirus 2 in Lake Biwa, Japan. The 59th Ecological Society of Japan, 2012.03.17-2012.03.21, Shiga, Japan. (in Japanese)

Takahara T, Doi H, Minamoto T, Kawabata Z, The biomass of fishes is presumed from the fragment of DNA dissolved all over the lake. The 59th Ecological Society of Japan, 2012.03.17-2012.03.21, Otsu, Japan. (in Japanese)

Minamoto T, Kawabata Z, Ecological analysis of infection: Koi herpes is made into an example. The forum of the infection by a youngman, 2012.02.02-2012.02.04, Nagasaki, Japan. (in Japanese)

Abe A, The ethical problem of the infection of an animal. The 30th Japanese Association for Philosophical and Ethical Researches, 2011.11.05-2011.11.06, Tokyo, Japan. (in Japanese)

Uchii K, Kawabata Z, The mechanism of propagation by the host of new pathogenic organ carp herpesvirus. The 27th Japanese Society of Microbial Ecology, 2011.10.08-2011.10.10, Kyoto, Japan. (in Japanese)

Takahara T, Honjo M, Minamoto T, Ito N, Kawabata Z, Is the incidence rate of a koi herpes virus disease subject to the influence of stress?. The Japanese Society of Applied Entomology and Zoology, 2011.03.27-2011.03.29, Fukuoka, Japan. (in Japanese)

Itayama T, Iwami N, Shimizu K, Furusawa F, Niwoot W, Sirapran F, Ruenkeaw P, Chayarat P, Korntip K, Sugiura N, Investigation of cyanobacterium which occurred in the culture pond in northern Thailand. The Japanese Society of Fisheries Science spring convention, 2011.03.27-2011.03.31, Shinagawa, Tokyo, Japan. (in Japanese)

Itayama T, Whangchai N, Chitmanat C, Kawabata Z, Investigation of influence in the fluvial environment and the farmed fish of the Ping of northern Thailand. The 45th Japan Society on Water Environment, 2011.03.18-2011.03.20, Sapporo, Hokkaido, Japan. (in Japanese)

Minamoto T, Yamanaka H, Honjo M, Kawabata Z, New method for fish biota survey using enviromental DNA. The 58th Ecological Society of Japan, 2011.03.08-2011.03.12, Sapporo, Hokkaido, Japan. (in Japanese)

Uchii K, Okuda N, Minamoto T, Kawabata Z, Influence of the wild type carp on Lake Biwa by a koi herpes virus disease. The 58th Ecological Society of Japan, 2011.03.08-2011.03.12, Sappro, Hokkaido, Japan. (in Japanese)

Minamoto T, Honjo M N, Yamanaka H, Uchii K, Kawabata Z, A Nation-wide survey for cyprinid herpesvirus-3 in Japan. The 75th Annual Meeting of the Japanese Society of Limnology, 2010.09.17-2010.09.20, Hirosaki City, Japan. (in Japanese)

Kawabata Z, Linkage of environment, KHV and humans. Workshop on the Linkage between CyHV-3(KHV) and Humans, 2010.05.13-2010.05.18, The Hebrew University, Jerusalem, Israel.

Minamoto T, Honjo M N, Kawabata Z, Seasonal dynamics of CyHV-3 in natural freshwater environments. Workshop on the Linkage between CyHV-3 (KHV) and Humans, 2010.05.13-2010.05.18, The Hebrew University, Jerusalem, Israel.

Itayama T, Koide M, Tanaka N, Yasukawa T, Matsue T, Kawabata Z, Development of microdevices and measurement methods in water environmental studies. Workshop on the Linkage between CyHV-3(KHV) and Humans, 2010.05.13-2010.05.18, The Hebrew University, Jerusalem, Israel.

Honjo M N, Minamoto T, and Kawanbata Z, Detection of cyprinid herpesvirus-3 (CyHV-3) in environmental water and sediments. Workshop on the Linkage between CyHV-3(KHV) and Humans, 2010.05.13-2010.05.18, The Hebrew University, Jerusalem, Israel.

Kawabata Z, Environmental diseases and Ecology. The 57th Ecological Society of Japan, 2010.03.17, Tokyo, Japan. (in Japanese)

Yamanaka H, Sogabe A, Omori K, Minamoto T, Miki T, Saito Y, Uchii K, Honjo M, Suzuki A, Komatsu Y, Kawabata Z, About the influence which spatiotemporal heterogeneity of behavioral temperature regulation and environmental temperature of a carp has on spread of a koi herpes virus disease. The 57th Ecological Society of Japan, 2010.03.15-2010.03.21, Tokyo, Japan. (in Japanese)

Uchii K, A technique indispensable to infection research. The 57th Ecological Society of Japan, 2010.03.15-2010.03.20, Tokyo, Japan. (in Japanese)

Yamamura N, Telschow A, Uchii K, Kawabata Z, Destruction of breeding space-population dynamics-pandemy of koi herpes. The 57th Ecological Society of Japan, 2010.03.15-2010.03.20, Tokyo, Japan. (in Japanese)

Minamoto T, Honjo M N, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Yonekura R, Omori K, Itayama T, Tanaka N, Asano K, Sirae Y, Okuda N, Kawabata Z, Interactions between koi herpesvirus and humans. (in Symposium S12: Linkage among environmental alteration, infectious diseases and humans). The 57th Annual Meeting of the Ecological Society of Japan, 2010.03.15-2010.03.20, Meguro, Tokyo, Japan. (in Japanese)

Honjo M N, Minamoto T, and Kawabata Z, Detection and quantification of cyprinid herpesvirus-3 (CyHV-3) from sediments in lake and pond. The 57th Annual Meeting of The Japanese Society of Ecology, 2010.03.15-2010.03.20, Meguro-ku, Tokyo. (in Japanese)

Uchii K, Telschow A, and Kawabata Z, Transmission mechanism of a viral pathogen through host reproductive behavior. 57th Annual Meeting of Japanese Society of Japan, 2010.03.15-2010.03.20, Tokyo. (in Japanese)

Tanaka N, Itayama T, Minamoto T, Honjo M, Kawabata Z, The system which condenses the virus in environmental water is developed. Japan Society on Water Environment, 2010.03.15-2010.03.17, Fukuoka Univ. (in Japanese)

Yamamura N, Uchii K, Kawabata Z, Telschow A, A basic equation for population dynamics with destruction of breeding habitats and its application to outbreak of KHV disease. The 25th Society of Population Ecology, 2009.10.17-2009.10.18, Doshisha Univ. (in Japanese)

Minamoto T, Honjo M N, Kawabata Z, Dynamics of the cyprinid herpesvirus 3 in Lake Biwa. The 74th Annual Meeting of the Japanese Society of Limnology, 2009.09.15-2009.09.17, Oita City, Japan. (in Japanese)

Yamanaka H, Sogabe A, Omori K, Minamoto T, Uchii K, Honjo M N, Suzuki A A, Kohmatsu Y, and Kawabata Z, Seasonality of behavioral thermoregulation in common carp: potential effects on the onset of Koi Herpesvirus disease. The Japanese Society of Limnology 74th annual meeting, 2009.09.14-2009.09.17, Ohita. (in Japanese)

Yamamura N, Telschow A, Uchii K, and Kawabata Z, A basic equation for population dynamics with destruction of breeding habitats and its application to outbreak of KHV disease.. 19th Annual Meeting of Japanese Society for Mathematical Biology, 2009.09.09-2009.09.11, Tokyo. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Rapid Detection of Respiratory Active Mycobacteria by Auramine O-CTC double staining. 30th Annual Congress European Society of Mycobacteriology, 2009.07.05-2009.07.08, Porto, Portugal.

Uchii K, Establishment of cyprinid herpesvirus 3 in a wild population of common carp in Lake Biwa. Workshop on CyHV-3 disease in an environment-human linkage, 2009.04.08, Kyoto.

Minamoto T, Honjo M N, Kawabata Z, Seasonal distribution of cyprinid herpesvirus 3 in Lake Biwa. Workshop on CyHV-3 disease in an environment-human linkage, 2009.04.08, Kyoto, Japan.

Minamoto T, Honjo M N, and Kawabata Z, Distribution of koi herpesvirus in Lake Biwa. The 56th Annual Meeting of The Japanese Society of Ecology, 2009.03.17-2009.03.21, Takizawa Village, Iwate. (in Japanese)

Yamanaka H, Kohmatsu Y, Minamoto T, Honjo M, Uchii K, Suzuki A, Kawabata Z, A lakefront form and water temperature distribution: The influence on fishes is considered. The 56th Ecological Society of Japan, 2009.03.17-2009.03.21, Iwate, Japan. (in Japanese)

Uchii K, Kawabata Z, Koi herpes virus (KHV) which is a foreign pathogenic microbe. The mechanism in which KHV is established to ordinary host population.. The 56th Ecological Society of Japan, 2009.03.17-2009.03.21, Iwate, Japan. (in Japanese)

Kakehashi M, The problem of Hominidae-livestock-pathogen system: Analysis of BSE-vCJD shows. A research meeting "the mathematical principle of the infection centering on avian flu - modeling and analysis - " , 2009.02.21-2009.02.22, Kyoto Sangyo University. (in Japanese)

Honjo N M, Minamoto T, Mastui K, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Iida T, Kawabata Z, Quantification of Koi herpesvirus in environmental water. 73th Annual Meeting of The Japanese Society of Limnology, 2008.10.11, Hokkaido. (in Japanese)

Uchii K, Ishihara T, Asano K, and Kawabata Z, Invasion of cyprinid herpesviru s 3 and its impact on the economy and industry involved in koi and common carp. 73rd Annual Meeting of Japanese Society of Limnology, 2008.10.11, Sapporo, Japan. (in Japanese)

Tanaka N, Itayama T, Honjo M, Minamoto T, Kawabata Z, Development of a Rapid Concentration System for Virus in Environmental water. 12th International Conference on Integrated Diffuse Pollution Management (IWA DIPCON 2008), 2008.08.27, Khon Kaen, Thailand.

Kawabata Z, Minamoto T, Honjo M N, Uchii K,Yamanaka H, Suzuki A A, and Kohmatsu Y, KHV-carp-human linkages: a case study in Lake Biwa, Japan. International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11-2008.06.12, Kyoto.

Tanaka N, Itayama T, Minamoto T, Honjo M, Kawabata Z, The quick method of condensing virus-like particles and detection of KHV. The 42th Japan Society on Water Environment, 2008.03.19-2008.03.21, Nagoya, Japan. (in Japanese)

Kawabata Z, Workshop: The bacteria in environment are detected quick and high-density. The 80th Japanese Society for Bacterioligy, 2007.03.26, Osaka, Japan. (in Japanese)

Kawabata Z, Asano K, Management of an ecological risk, and regeneration of nature. Specific domain research launched in the Heisei 18 fiscal year "Multistory environmental governance of sustainable development" symposium, 2007.03.21, Kyoto, Japan. (in Japanese)

Yamaguchi N, Baba T, Maruyama K, Tani K, and Nasu M, Rapid enumeration of active Legionella pneumophila in aquatic environments by microcolony method. 11th International Symposium on Microbial Ecology, 2006.08.21, Vienna.

Kawabata Z, Preliminary research of a RIHN project: The interaction ring of "Environmental change of man and freshwater environment" and "infection". The large-scale research project of a forum: A tendency and a measure. The 53th Ecological Society of Japan, 2006.03.25, Niigata, Japan. (in Japanese)

【Poster Presentation】

Kato T, Ichijo T, Izumi Y, Nakamoto S, Yamaguchi N, Nasu M, Distribution of culturable Mycobacterium avium and its subspecies in residences. International Union of Microbiological Societies 2011 Congress (IUMS2011), 2011.09.06-2011.08.10, Hokkaido, Japan.

Ichijo T, Izumi Y, Nakamoto S, Yamaguchi N, Nasu M, Dynamics of nontuberculous mycobacteria in residential environments determined by culture-independent methods. International Union of Microbiological Societies 2011 Congress (IUMS2011), 2011.09.06-2011.09.10, Hokkaido, Japan.

Minamoto T, Honjo M N, Kawabata Z, Dynamics of Cyprinid herpesvirus 3 in natural environments in Japan. 4th Congress of European Microbiologists, FEMS 2011, 2011.06.26-2011.06.30, Geneva, Switzerland.

Ichijo T, Izumi Y, Nakamoto S, Yamaguchi N, Nasu M, Dynamics of Mycobacterium avium complex in residential environments determined by culture-independent methods. 32nd Annual Congress of the European Society of Mycobacteriology, 2011.06.26-2011.06.29, Lübeck, Germany.

Ichijo T, Izumi Y, Nakamoto S, Yamaguchi N, Nasu M, Dynamics of Mycobacteria in Housing Environment Determined with Culture-independent Approach. 111th General Meeting American Society for Microbiology, 2011.05.21-2011.05.24, New Orleans, USA.

Yamanaka H, Minamoto T,Takahara T, Kawabata Z, Physiological cost of fish caused by temperature fluctuation. 58th Ecological Society of Japan Annual Meeting, 2011.03.08-2011.03.12, Sapporo. (in Japanese)

Takahara T, Yamanaka H, Suzuki A, Honjo M, Minamoto T, Yonekura R, Itayama T, Kohmatsu Y, Ito N, Kawabata Z, The trade-off with bank environment important for a carp and the evasion method of the stress by water temperature change in the daytime is considered. The 58th Ecological Society of Japan, 2011.03.08-2011.03.12, Sapporo, Japan. (in Japanese)

Yamanaka H, Minamoto T, Wu D, Kong H, Wei Z-h, Liu B, Kawabata Z, Spatiotemporal distribution of water temperature in Lake Erhai, Yunnan, China. The 75th Annual Meeting of the Japanese Society of Limnology, 2010.09.17-2010.09.20, Hirosaki City, Japan. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Application of Auramine O-CTC double staining method for rapid detection of respiratory active MAC (Mycobacterium avium complex). Forum2010: Pharmaceutical Health Science・Environmental Toxicology, 2010.09.09-2010.09.10, Tokyo, Japan. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Rapid Enumeration of Respiratory Active Mycobacteria with Fluorescent Double Staining. 13th International Symposium on Microbial Ecology, 2010.08.22-2010.08.27, Seattle, WA, USA.

Yamaguchi N, Uebayashi Y, Torii M, Nasu M, Rapid Monitoring of Legionella pneumophila in Aquatic Environment by Using a Microfluidic Device. 13th International Symposium on Microbial Ecology, 2010.08.22-2010.08.27, Seattle, WA, USA,.

Yamaguchi N, Uebayashi Y, Torii M, Nasu M, Rapid Enumeration of Legionella pneumophila in Aquatic Environment by Using a Microfluidic Device. 110th General Meeting of America Society for Microbiology, 2010.05.23-2010.05.27, San Diego, CA, USA.

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Rapid Detection of Respiratory Active Mycobacteria by Multicolor Imaging. 110th General Meeting of America Society for Microbiology, 2010.05.23-2010.05.27, San Diego, CA, USA.

Ichijo T, Hiramatsu A, Kenzaka T, Baba T, Yamaguchi N, Nasu M, Diversity of Legionella pneumophila in aquatic environments. The 130th Annual Meeting of the Pharmaceutical Society of Japan, 2010.03.28-2010.03.30, Okayama. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Auramine O-CTC double staining as a method for rapid detection of respiring Mycobacteria. The 130th Annual Meeting of the Pharmaceutical Society of Japan, 2010.03.28-2010.03.30, Okayama. (in Japanese)

Yamaguchi N, Nasu M, Relationship between bacteria and human in aquatic environments. The 83rd Annual Meeting of Japanese Society for Bacteriology, 2010.03.27-2010.03.29, Yokohama, Kanagawa. (in Japanese)

Ichijo T, Yamaguchi N, Nasu M, Rapid detection of respiratory active nontuberculous mycobacteria by Auramin e O-CTC double staining. The 83rd Annual Meeting of Japanese Society for Bacteriology, 2010.03.27-2010.03.29, Yokohama, Kanagawa. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, and Nasu M, Rapid detection of respiratory active Mycobacteria by Auramine O – CTC double staining. 25th JSME Annual Meeting, 2009.11.21-2009.11.23, Higashi-Hiroshima, Hiroshima. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, and Nasu M, Rapid detection of respiratory active non-tuberculous mycobacteria by Auramine O-CTC double staining. Forum 2009: Pharmaceutical Health Science and Environmental Toxicology, 2009.11.05-2009.11.06, Ginowan, Okinawa. (in Japanese)

Kawabata Z, Destruction of littoral zone, koi herpesvirus, and human linkages: A case study of Lake Biwa, Japan. DIVERSITAS Open Science Conference(DIVERSITAS OS2), 2009.10.14-2009.10.16, Cape Town, South Africa.

Ichijo T, Baba T, Inoue N, Kenzaka T, Yamaguchi N, Nasu M, Diversity of Legionella pneumophila isolated from aquatic environments determined by genotyping. 62nd Annual Meeting of Japanese Society of Hot Spring Sciences, 2009.09.09-2009.09.10, Kyoto. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, and Nasu M, Rapid Detection of Respiratory Active Mycobacteria by Auramine O-CTC Double Staining. 30th Annual Congress European Society of Mycobacteriology, 2009.07.05-2009.07.08, Porto, Portugal.

Matsumae H, Minamoto T, Hanai S, Ishiwata R, Izumi K, Ogishima S, Tanaka H, Satoh N, Ishida N, Microarray analysis of circadian gene expressions in Ciona intestinalis. The 5th International Tunicate Meeting, 2009.06.21-2009.06.25, Naha City, Okinawa, Japan.

Ichijo T, Kenzaka T, Inoue N, Hiramatsu A, Baba T, Yamaguchi N, Nasu M, Genetic Difference of Legionella pneumophila Isolates between from Natural and from Anthropogenic Environments. 109th American Society for Microbiology General Meeting, 2009.05.17-2009.05.22, Philadelphia, PA, USA.

Ichijo T, Inoue N, Kenzaka T, Yamaguchi N, Nasu M, Diversity of the Legionella pneumophila in environmental water. The 129th Pharmaceutical Society of Japan, 2009.03.26-2009.03.28, Kyoto, Japan. (in Japanese)

Ichijo T, Izumi Y, Yamaguchi N, Nasu M, Specific detection of an acid-proof bacterium with the respiration activity by an Auramine O-CTC double stain. The 129th Pharmaceutical Society of Japan, 2009.03.26-2009.03.28, Kyoto, Japan. (in Japanese)

Yamanaka H, Kohmatsu Y,Minamoto T, Honjo M, Uchii K, Suzuki A, Kawabata Z, A lakefront form and water temperature distribution: Consideration of influence to fishes. The 56th Ecological Society of Japan, 2009.03.17-2009.03.21, Iwate, Japan. (in Japanese)

Ichijo T, Baba T, Kenzaka T, Yamaguchi N, Nasu M, The genotype of the quality of membranal protein of Legionella pneumophila in water environment . The 82th Japanese Society for Bacterioligy, 2009.03.12-2009.03.14, Nagoya, Japan. (in Japanese)

Matsui K, Honjo N M, Kawabata Z, Uchii K, Use of bacterial horizontal gene transfer event to characterize freshwater environments with different trophic status. 24th the Japanese Society of Microbial Ecology Annual Meeting, 2008.11.25-2008.11.28, Hokkaido, Japan. (in Japanese)

Ichijo T, Yamaguchi N, Nasu M, Simultaneous detection of the bacteria in the water environment by the bead assay method. The 24th Japanese Society of Microbial Ecology, 2008.11.25-2008.11.28, Sapporo, Japan. (in Japanese)

Baba T, Inoue N, Yasui M, Ichijo T, Kenzaka T, Nasu M, Genetic diversity of membrane protein gene sequences in Legionella pneumophila isolated from natural and artificial environments. The 12th International Symposium on Microbial Ecology, 2008.10.18, Cairns, Australia.

Ichijo T, Yamaguchi N, Tani K, and Nasu M, Beads Assay Based Simultaneous Detection of Pathogenic Bacteria in Aquatic Environment. 12th International Symposium on Microbial Ecology, 2008.08.17-2008.08.22, Cairns, Australia.

Minamoto T, Honjo M N, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, and Kawabata Z, Detection of koi herpesvirus DNA from natural river water. International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11-2008.06.12, Kyoto, Japan.

Suzuki A, Kohmatsu Y, Yonekura R, Measuring cortisol in the water as an indicator of stress caused by environmental change in common carp(Cyprinus carpio). International Symposium, Environmetal Change, Pathogens, and Human Linkages, 2008.06.11, Kyoto, Japan.

Yamanaka H, Sogabe A, Kohmatsu Y, Minamoto T, Honjo N M, Uchii K, Suzuki A A, Omori K, Kawabata Z, Relationship of lake morphometry and shore configuration to the temperature distribution inlagoons, and implications for its effect on fish health. International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11, Kyoto.

Itayama T, Yanaka N, Honjo N M, Minamoto T, Kawabata Z, Development of an on site rapid concentration system for virus in environmental water. International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11-2008.06.12, Kyoto, Japan.

Itayama T, Tanaka N, Honjo M N, Minamoto T, and Kawabata Z, Development of an on site rapid concentration system for virus in environmental water. International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11-2008.06.12, Kyoto, Japan. (in Japanese)

Ichijo T, Yamaguchi N, Nasu M, Diversity of eukaryotic-like gene sequences in legionella pneumophila isolated from aquatic environment in Asian countries by using molecular microbial ecological methods. International Symposium, Environmetal Change, Pathogens, and Human Linkages, 2008.06.11, Kyoto.

Honjo N M, Minamoto T, Matsui K, Uchii K, Yamanaka H, Suzuki A A, Kohmatsu Y, Iida T, Kawabata Z, Quantification method of Koi herpesvirus (KHV) in environmental water using cation-coated filtermethod and external standard virus. International Symposium on Environmental Change, Pathogens, and Human Linkages , 2008.06.11, Kyoto, Japan.

Uchii K, Matsui K, and Kawabata Z, Distribution of cyprinid herpesvirus 3 in a wild population of common carp (Cyprinus carpio). International Symposium on Environmental Change, Pathogens, and Human Linkages, 2008.06.11-2008.06.12, Kyoto,Japan.

Kenzaka T, Yasui M, Ichijo T, Baba T, Nasu M, Diversity of Eukaryotic-Like Gene Sequences in Legionella pneumophila Isolated from Natural Environment. 108th American Society for Microbiology General Meeting,, 2008.06.01-2008.06.05, Boston, MA, USA.

Itayama T, Koide M, Matsue T, Yasukawa T, Kawabata Z, Ichimura N, Fujii Y, Development of the technology which conducts performance analysis of the microbe new for environmental pollution restoration quickly. nano tech 2012 International Nanotechnology Exhibition & Conference, 2007.02.21-2007.02.23, Tokyo, Japan. (in Japanese)

【Invited Lecture / Honorary Lecture / Panelist】

Minamoto T, Environmental change and illness of a fish. 10th RIHN inter-regional association seminar - Possibility of preservation of the waterside, and the future of Lake Biwa -, 2012.01.14, Shiga, Japan. (in Japanese)

Minamoto T, and the members of RIHN C-06 project Koi herpesvirus disease as a model of environmental disease. The 6th RIHN International Symposium, 2011.10.26-2011.10.28, Kyoto, Japan.

Uchii K, Telschow A, Okuda N, Minamoto T, Yamanaka H, Honjo MN, Matsui K, Kawabata Z, Transmission dynamics of KHV and its impact on the genetic structure of the host population. International Symposium on Emergence of Viral Diseases: Evolution and Ecology of Koi Herpes Virus, 2011.07.04-2011.07.05, Munster, Germany.

Minamoto T, Honjo M N, Yamanaka H, Uchii K, Kawabata Z, KHV dynamics in natural freshwater environments. Emergence of Viral Diseases: Ecology and Evolution of Koi Herpes Virus, 2011.06.04-2011.06.05, Muenster, Germany.

Itayama T, Whangchai N, Chitmanat C, Iwami N, Fakrajang S, Pleumsumran C, Praphrute R, Vuthijumnonk J, Shimizu K, Sugiura N, Kawabata Z, Water Environment, Human activity and Aquaculture, in The Third International Conference with the Subject: Effect of Human Activities and on Fisheries Resources. Maejo University, 2010.12.08-2010.12.09, ChangMai, Thailand.

Kawabata Z, Environmental Diseases- A New strategy of ecosystem conservation. The 16th Symposium on How to Prevent Infectious Diseases from the View Point of Conservation Medicine, 2010.09.02, Japanese Society of Zoo and Wild Life Medicine, Fukuoka. (in Japanese) Abstruct p25

Minamoto T, Molecular evolution of diversity of fishes and visual pigment. Zoological Society of Japan in the Heisei 22 fiscal year, 2010.07.24, Gifu, Japan. (in Japanese)

Itayama T, Kawabata Z, Impact of human activity for fish diseases A case study on Tilapia aquaculture, aculty of Fisheries Technology and Aquatic Resource. Maejyo University, 2010.06.07, Cheng Mai, Thailand.

Kawabata Z, KHV and human linkage. Faculty of Fisheries Technology and Aquatic Resource.Maejyo University, 2010.06.07, Cheng Mai, Thailand.

Yamaguchi N, Nasu M, Relationship between bacteria and human in aquatic environments. The 83rd Annual Meeting of Japanese Society for Bacteriology, 2010.03.27-2010.03.29, Yokohama, Kanagawa. (in Japanese)

Nasu M, Ichijo T, Environmental diseases. 57th Annual Meeting of Ecological Society of Japan, 2010.03.15-2010.03.20, Meguro, Tokyo. (in Japanese)

Kakehashi M, Infectious diseases induced by human activities: Pandemic influenza, BSE and drug resistant bacteria. Symposium: The connection between environmental change, infectious diseases and humans, The Annual Meeting of the Ecological Society of Japan, 2010.03.15-2010.03.20, Tokyo. (in Japanese)

Minamoto T, Honjo M, Uchii K, Yamanaka H, Suzuki A, Kohmatsu Y, Yonekura R, Omori K, Itayama T, Tanaka N, Asano K, Shirae Y, Okuda N, Kawabata Z, The interaction ring of koi herpesvirus infection and a human being. The 57th Ecological Society of Japan, 2010.03.15-2010.03.20, Tokyo, Japan. (in Japanese)

Itayama T, Aquatic environment and new technologies in Japan. The second International Conference with the Subject: Attaining Security of Fisheries and Aquatic Resources, 2009.12.08-2009.12.10, Maejo University, Chiangmai Thailand. Key note presentation

Itayama T, Microbe activity sensing using a micro fluid system. Japan Electronics and Information Technology Industries Association: Sensing device technology subcommittee, 2009.09.25, National Institute for Environmental Studies. (in Japanese)

Kawabata Z, Koi herpesvirus disease as an infection model: preservation - how with the infection based on a medical idea to associate . Japanese Society of Zoo and Wildlife Medicine, 2009.09.04, Toyama, Tokyo. (in Japanese)

Kawabata Z, Environmental change, pathogen, and human linkage :A case study of Lake Biwa, Japan. Special Symposium, Aquatic Ecosystem Restoration, World City Water Forum 2009(WCWF2009), 2009.08.21, Inchon, Korea.

Kawabata Z, Concept of environmental disease. Dali University, 2009.06.25, Dali, Yunnan, China.

Kawabata Z, KHV-Carp-Human Linckages: case study in Lake Biwa, Japan. Seminar on Environmental Diseases. Lecture at Graduate School of Pharmaceutical Sciences, Osaka University, 2008.10.29, Osaka. (in Japanese)